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Int Neurourol J > Volume 24(2); 2020 > Article
Lee: Commentary on “The Urine Microbiome of Healthy Men and Women Differs by Urine Collection Method”
To the editor,
I read with great interest the article titled “The Urine Microbiome of Healthy Men and Women Differs by Urine Collection Method” published by Pohl et al. in the International Neurourology Journal in March 2020 [1]. The authors concluded that the urinary microbiome differed according to urine collection methods and that sex differences in the core microbiome exist.
When I analyzed articles that studied urinary microbiome in healthy individuals, or healthy controls in case-control studies, most studies showed similar results (Table 1). Lactobacillus was the predominant genus in the urinary microbiome in females, whereas a more heterogeneous group of microbiomes was shown in males [2-23]. The study by Wolfe et al. [5] was the only study that compared different urine collection methods, and they concluded that the best methods are suprapubic aspiration and transurethral catheterization.
There are still clear limitations in the study of the human urinary microbiome. Due to the heterogeneous design of urinary microbiome studies, it is difficult to make comparisons between studies and to draw conclusions. There are differences according to the urine collection method, 16s rRNA analysis tools, statistical methods, and the taxonomic database on which the reporting of genera or species is based. These heterogeneous study designs cause discrepancies in results. In addition, as the authors clearly pointed out, many other factors can influence the urinary microbiome, such as age, sex, dietary habits, infections and antimicrobial use, hormonal status, and regional variation.
Several points should be considered in future studies involving the urinary microbiome. First, a larger sample size is needed. Second, standardization of methodology and reporting is necessary to facilitate comparisons between studies. Third, more studies on the normal urinary microbiome and factors influencing its composition are needed. Age, sex, and urine collection methods are known factors, but many other factors likely exist. One point to consider in this regard is that it may be impossible to define a “universal norm” regarding the urinary microbiome. Rather than defining a universal norm, identifying the normal urinary microbiome in each individual and using it as a personalized reference for future disease may be a more reasonable approach. Fourth, the connection between dysbiosis and disease should be more clearly identified. When this connection is found, it may become possible to use the urinary microbiome in diagnosis or treatment. Lastly, studies have suggested that the overall composition and richness of the microbiota play an important role in modulating vaccine response [24]. In this regard, the effects of the urinary microbiome on vaccinations for urinary tract infection may be an interesting topic for future studies.

NOTES

Conflict of Interest
No potential conflict of interest relevant to this article was reported.

REFERENCES

1. Pohl HG, Groah SL, Perez-Losada M, Ljungberg I, Sprague BM, Chandal N, et al. The urine microbiome of healthy men and women differs by urine collection method. Int Neurourol J 2020;24:41-51. PMID: 32252185
crossref pmid pmc pdf
2. Siddiqui H, Nederbragt AJ, Lagesen K, Jeansson SL, Jakobsen KS. Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons. BMC Microbiol 2011;11:244. crossref
3. Fouts DE, Pieper R, Szpakowski S, Pohl H, Knoblach S, Suh MJ, et al. Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury. J Transl Med 2012;10:174. PMID: 22929533
crossref pmid pmc pdf
4. Nelson DE, Dong Q, Van der Pol B, Toh E, Fan B, Katz BP, et al. Bacterial communities of the coronal sulcus and distal urethra of adolescent males. PLoS One 2012;7:e36298. PMID: 22606251
crossref pmid pmc
5. Wolfe AJ, Toh E, Shibata N, Rong R, Kenton K, Fitzgerald M, et al. Evidence of uncultivated bacteria in the adult female bladder. J Clin Microbiol 2012;50:1376-83. PMID: 22278835
crossref pmid pmc
6. Lewis DA, Brown R, Williams J, White P, Jacobson SK, Marchesi JR, et al. The human urinary microbiome; bacterial DNA in voided urine of asymptomatic adults. Front Cell Infect Microbiol 2013;3:41. PMID: 23967406
crossref pmid pmc
7. Hilt EE, McKinley K, Pearce MM, Rosenfeld AB, Zilliox MJ, Mueller ER, et al. Urine is not sterile: use of enhanced urine culture techniques to detect resident bacterial flora in the adult female bladder. J Clin Microbiol 2014;52:871-6. PMID: 24371246
crossref pmid pmc
8. Pearce MM, Hilt EE, Rosenfeld AB, Zilliox MJ, Thomas-White K, Fok C, et al. The female urinary microbiome: a comparison of women with and without urgency urinary incontinence. mBio 2014;5:e01283-14. PMID: 25006228
crossref pmid pmc
9. Karstens L, Asquith M, Davin S, Stauffer P, Fair D, Gregory WT, et al. Does the urinary microbiome play a role in urgency urinary incontinence and its severity? Front Cell Infect Microbiol 2016;6:78. PMID: 27512653
crossref pmid pmc
10. Thomas-White KJ, Hilt EE, Fok C, Pearce MM, Mueller ER, Kliethermes S, et al. Incontinence medication response relates to the female urinary microbiota. Int Urogynecol J 2016;27:723-33. PMID: 26423260
crossref pmid pdf
11. Abernethy MG, Rosenfeld A, White JR, Mueller MG, Lewicky-Gaupp C, Kenton K. Urinary microbiome and cytokine levels in women with interstitial cystitis. Obstet Gynecol 2017;129:500-6. PMID: 28178051
crossref pmid
12. Gottschick C, Deng ZL, Vital M, Masur C, Abels C, Pieper DH, et al. The urinary microbiota of men and women and its changes in women during bacterial vaginosis and antibiotic treatment. Microbiome 2017;5:99. PMID: 28807017
crossref pmid pmc pdf
13. Rani A, Ranjan R, McGee HS, Andropolis KE, Panchal DV, Hajjiri Z, et al. Urinary microbiome of kidney transplant patients reveals dysbiosis with potential for antibiotic resistance. Transl Res 2017;181:59-70. PMID: 27669488
crossref pmid
14. Wang H, Altemus J, Niazi F, Green H, Calhoun BC, Sturgis C, et al. Breast tissue, oral and urinary microbiomes in breast cancer. Oncotarget 2017;8:88122-38. PMID: 29152146
crossref pmid pmc
15. Wu P, Chen Y, Zhao J, Zhang G, Chen J, Wang J, et al. Urinary microbiome and psychological factors in women with overactive bladder. Front Cell Infect Microbiol 2017;7:488. PMID: 29230385
crossref pmid pmc
16. Bucevic Popovic V, Situm M, Chow CT, Chan LS, Roje B, Terzic J. The urinary microbiome associated with bladder cancer. Sci Rep 2018;8:12157. PMID: 30108246
crossref pmid pmc
17. Komesu YM, Richter HE, Carper B, Dinwiddie DL, Lukacz ES, Siddiqui NY, et al. The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls. Int Urogynecol J 2018;29:1785-95. PMID: 29909556
crossref pmid pmc pdf
18. Wu P, Zhang G, Zhao J, Chen J, Chen Y, Huang W, et al. Profiling the urinary microbiota in male patients with bladder cancer in China. Front Cell Infect Microbiol 2018;8:167. PMID: 29904624
crossref pmid pmc
19. Bresler L, Price TK, Hilt EE, Joyce C, Fitzgerald CM, Wolfe AJ. Female lower urinary tract microbiota do not associate with IC/PBS symptoms: a case-controlled study. Int Urogynecol J 2019;30:1835-42. PMID: 30993388
crossref pmid pdf
20. Kassiri B, Shrestha E, Kasprenski M, Antonescu C, Florea LD, Sfanos KS, et al. A Prospective study of the urinary and gastrointestinal microbiome in prepubertal males. Urology 2019;131:204-10. PMID: 31195012
crossref pmid
21. Meriwether KV, Lei Z, Singh R, Gaskins J, Hobson DTG, Jala V. The vaginal and urinary microbiomes in premenopausal women with interstitial cystitis/bladder pain syndrome as compared to unaffected controls: a pilot cross-sectional study. Front Cell Infect Microbiol 2019;9:92. PMID: 31024861
crossref pmid pmc
22. Liu F, Zhang N, Jiang P, Zhai Q, Li C, Yu D, et al. Characteristics of the urinary microbiome in kidney stone patients with hypertension. J Transl Med 2020;18:130. PMID: 32183836
crossref pmid pmc pdf
23. Xie J, Huang JS, Huang XJ, Peng JM, Yu Z, Yuan YQ, et al. Profiling the urinary microbiome in men with calcium-based kidney stones. BMC Microbiol 2020;20:41. PMID: 32111156
crossref pmid pmc pdf
24. Ciabattini A, Olivieri R, Lazzeri E, Medaglini D. Role of the microbiota in the modulation of vaccine immune responses. Front Microbiol 2019;10:1305. PMID: 31333592
crossref pmid pmc

Table 1.
Articles that studied the urinary microbiome in healthy individuals, or healthy controls in case-control studies
Study No. Sex Age Urine collection Microbiome
Siddiqui et al. [2] (2011) 8 F 27-67 yr CCU Lactobacillus, Prevotella, Gardnerella, Peptoniphilus, Dialister, Finegoldia, Anaerococcus, Allisonella, Streptococcus, Staphylococcus
Wolfe et al. [5] (2012) 12 F NA CCU, TUC, SPA Lactobacillus, Actinobaculum, Aerococcus, Anaerococcus, Atopobium, Burkholderia, Corynebacterium, Gardnerella, Prevotella, Ralstonia, Sneathia, Staphylococcus, Streptococcus, Veillonella
Fouts et al. [3] (2012) 11 M 24-50 yr MSU Corynebacterium, Staphylococcus, Streptococcus, Lactobacillus, Gardnerella, Veillonella
15 F 22-51 yr Lactobacillus, Corynebacterium, Staphylococcus, Streptococcus, Prevotella
Nelson et al. [4] (2012) 18 M 14-17 yr FCU Corynebacterium, Lactobacillus, Staphylococcus, Gardnerella, Streptococcus, Anaerococcus, Veillonella, Prevotella, Escherichia
Lewis et al. [6] (2013) 6 M 39-83 yr CCU Firmicutes
10 F 26-90 yr Firmicutes, Actinobacteria, Bacteroidetes
Hilt et al. [7] (2014) 24 F NA TUC Lactobacillus, Corynebacterium, Streptococcus, Actinomyces, Staphylococcus, Aerococcus, Gardnerella, Bifidobacterium, Actinobaculum
Pearce et al. [8] (2014) 58 F 35-65 yr TUC Lactobacillus, Gardnerella, Corynebacterium, Enterobacteriaceae, Anaerococcus, Bifidobacterium, Streptococcus, Staphylococcus, Sneathia, Peptoniphilus, Atopobium, Rhodanobacter, Trueperella, Alloscardovia, Veillonella
Karstens et al. [9] (2016) 10 F 58 (mean) TUC Anoxybacillus, Lactobacillus, Prevotella, Gardnerella, Arthrobacter, Escherichia, Shigella
Thomas-White et al. [10] (2016) 60 F 35-65 yr TUC Lactobacillus, Gardnerella, Staphylococcus, Streptococcus, Enterococcus, Bifidobacterium, Atopobium, Enterobacteriaceae
Wu et al. [15] (2017) 25 F 26 (mean) TUC Lactobacillaceae, Prevotellaceae, Enterobacteriaceae, Veillonellaceae, Tissierellaceae, Bifidobacteriales
Gottschick et al. [12] (2017) 49 F 19-62 yr MSU Lactobacillus crispatus
Abernethy et al. [11] (2017) 20 F 28-43 yr TUC Lactobacillus acidophilus
Wang et al. [14] (2017) 21 F 43 (mean) MSU Lactobacillus, Varibaculum, Porphyromonas, Prevotella, Bacteroides
Rani et al. [13] (2017) 5 F 27-63 yr MSU Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes
3 M
Wu et al. [18] (2018) 18 M 55.5 (mean) MSU Escherichia-Shigella, Staphylococcus, Streptococcus, Aeromonas, Acinetobacter, Bacteroides, Lactobacillus
Komesu et al. [17] (2018) 84 F 53 (mean) TUC Lactobacillus, Gardnerella, Tepidimonas, Prevotella
Bucevic Popovic et al. [16] (2018) 19 M 61-82 yr MSU Firmicutes, Actinobacteria, Bacteroidetes, Proteobacteria
Meriwether et al. [21] (2019) 18 F 33.7 (mean) MSU Lactobacillus, Prevotella
Bresler et al. [19] (2019) 20 F 48 (mean) MSU Lactobacillus
Kassiri et al. [20] (2019) 10 M 3 mo-8 yr TUC Staphylococcus, Varibaculum, Peptoniphilus, Actinobaculum
Xie et al. [23] (2020) 21 M 44.2 (mean) NA Acinetobacter, Prevotella, Oscillospira, Parabacteroides, Fusobacterium
Liu et al. [22] (2020) 9 M 58.9 (mean) TUC Gardnerella, Pontibacter, Sphingomonas, Prevotella, Propionibacterium
3 F

CCU, clean catch urine; TUC, transurethral catheter; SPA, suprapubic aspirate; MSU, midstream urine; FCU, first catch urine; NA, not available.

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